PLANT RESISTANCE Limited Genetic Variation Within and Between Russian Wheat Aphid (Hemiptera: Aphididae) Biotypes in the United States

Insect biotypes are populations able to kill or injure crops with resistance genes and complicate pest management programs based on host plant resistance. Biotypes occur in Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae), a worldwide pest of wheat, Triticum aestivum L., and barley, Hordeum vulgare L., that was introduced into Mexico in 1980 and then spread into Texas by 1986. FiveD. noxia biotypes were described in the United States and given the number designations 1 through 5. Of these, only Biotypes 1 and 2, which are nondamaging and damaging toDn4-resistantwheat, respectively, are commonandagriculturally important.Only a single clone of Biotypes 3, 4, and 5 were found in nature and now exist in the laboratory. The biotypes were found after 5 yr of the commercial planting of resistant wheat and their origin is unknown. To understand the genetic relatedness and origin ofD. noxia biotypes in the United States, we used three molecularmarkers to assay for genetic variationwithin andbetweenBiotypes 1 and2, and for variation between Biotypes 1, 2, 3, 4, and 5. A single random ampliÞed polymorphic DNA polymorphism was found in only two individuals. No DNA sequence variation in the cytochrome oxidase subunit I mitochondrial gene was found between 26D. noxia clones. No variation was found at seven examined simple sequence repeat loci. Results suggest Biotype 2 originated from the extant population and does not represent a second introduction of a genetically divergent biotype.